FastQCFastQC Report
Fri 1 Mar 2019
H3537BGXB_n01_HFL36.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3537BGXB_n01_HFL36.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4826211
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG612811.2697538503807646No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA280610.5814291998422779No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATCA267800.5548866388145898No Hit
TCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCAT230050.47666792852612533No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT206950.4288042938860319No Hit
CTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATC189690.39304124912897503No Hit
GATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACC172440.35729892456007417No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG163500.3387750763487133No Hit
TTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATCAAT153100.31722608066659336No Hit
CGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCAC136680.2832035317146308No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG127420.26401663748228166No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC102590.21256841029122017No Hit
TGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGC99310.20577218857609No Hit
CTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGG93030.1927599104141945No Hit
ATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCA88440.18324934405064347No Hit
GCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAACTG82610.17116947435576274No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC77680.1609544215949116No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATCAA65120.13492986527112055No Hit
CCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACT58180.12055005469093663No Hit
CGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAACTGT54380.1126763831917005No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTAACTGTAGGCACCATCA51540.10679184975542927No Hit
CCCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAC50640.10492703282139965No Hit
GAGCAGCTCCCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGG48950.10142532102305514No Hit
GCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCA48270.10001634822845498No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGGT262650.069.250687
AGGTTGT277400.069.2097710
GTAGGTT261000.069.2064748
TAGGTTG261400.069.08729
GGTAGTA351300.069.073634
TGAGGTA364000.069.047461
GTTGTGT82800.069.0030212
GTTGTAT197250.068.9857912
TAGTAGG261600.068.982386
GAGGTAG372450.068.784322
GTGTGGT81550.068.7323415
GGTTGTG83150.068.6719711
TATAGTT212500.068.6066316
GTAGTAG352200.068.551285
TGTATAG243400.068.5110414
CTGAGAC237000.068.282144
TTGTATA244300.068.23003413
TGTGGTT75100.068.21419516
TCCCTGA236600.068.153621
AGACCCT237350.068.048947
Produced by FastQC (version 0.11.5)