FastQCFastQC Report
Fri 1 Mar 2019
H3537BGXB_n01_HFL35.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3537BGXB_n01_HFL35.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5941151
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG466700.7855380211679521No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATCA351900.592309470000005No Hit
TCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCAT293940.4947526161176513No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA279030.4696564689232776No Hit
CTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATC234150.39411555101023354No Hit
GATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACC229320.38598581318670405No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT214510.36105798354561264No Hit
TTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATCAAT212160.3571025210434813No Hit
CGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCAC194420.3272429870912219No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG170910.2876715303145805No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG153110.2577110058303517No Hit
TGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGC143740.2419396510878111No Hit
GCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAACTG142260.23944855129923479No Hit
CTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGG138490.23310297954049647No Hit
ATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCA138480.23308614778516823No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATCAA104800.1763967958397287No Hit
CGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAACTGT104370.1756730303606153No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC95780.1612145525336757No Hit
CCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACT87980.14808578337766537No Hit
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAACT86490.14557785183376082No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC79080.13310552113555102No Hit
CCCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAC75610.12726490203665924No Hit
GCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCA65440.11014700686786112No Hit
GAGCAGCTCCCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGG64530.10861531713299326No Hit
CGCACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCG60820.10237073590622424No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT216150.069.1509558
AGGTTGT230500.069.13560510
TAGGTTG215950.069.13419
AGTAGGT218300.069.0289157
GGTAGTA283650.069.0178454
GGTTGTG68700.068.8600411
GTTGTAT163350.068.8088812
GAGGTAG301050.068.801322
TGAGGTA296500.068.779141
GTTGTGT68700.068.7594812
TAGTAGG217500.068.7103046
GTGTGGT68250.068.2890715
TATAGTT175600.068.2842916
TGTATAG198500.068.02788514
GCTACAT63850.068.0201342
GTAGTAG287150.068.0056845
TTGTATA199200.067.8759613
TTGTGTG69650.067.7686113
GTATAGT199350.067.5800915
TGTGGTT63000.067.546816
Produced by FastQC (version 0.11.5)