FastQCFastQC Report
Fri 1 Mar 2019
H3537BGXB_n01_HFL31.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3537BGXB_n01_HFL31.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4784383
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG486341.0165156092227565No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA278380.5818514111432969No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATCA229730.4801664080822961No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT202380.42300125219908186No Hit
TCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCAT193020.4034376010448996No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG168130.3514141739906692No Hit
GATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACC157740.3296976851560588No Hit
CTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATC154040.3219641905758799No Hit
TTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATCAAT138680.28985973739978593No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG138160.28877286789122025No Hit
CGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCAC126470.2643392052851956No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC119360.24947835488923023No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC99500.20796830019670248No Hit
TGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGC96800.20232493928684223No Hit
CTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGG95230.19904342942444198No Hit
GCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAACTG92090.19248040969964153No Hit
ATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCA88510.18499773115990087No Hit
CGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAACTGT67880.14187827354122778No Hit
CCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACT67760.14162745750078953No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATCAA66180.13832504630168613No Hit
CCCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAC61490.12852231938789183No Hit
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAACT54290.11347335696159776No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGGT233900.069.171937
GTAGGTT232600.069.137968
TAGGTTG232650.069.0766149
AGGTTGT245750.069.0763910
GGTAGTA306850.069.02584
TGAGGTA316050.068.935291
TAGTAGG233150.068.92076
GTTGTAT170900.068.7664412
GTTGTGT77250.068.73971612
GGTTGTG77500.068.6539311
TATAGTT185050.068.643916
GAGGTAG324950.068.621772
GCTACAT60000.068.5404362
GTAGTAG307850.068.529195
GTGTGGT75850.068.3964415
TGTATAG207350.068.337114
TTGTATA207300.068.30302413
AGACCCT185950.068.276947
TGAGACC186700.068.083215
TGCTGGG50950.068.0189813
Produced by FastQC (version 0.11.5)