FastQCFastQC Report
Fri 1 Mar 2019
H3537BGXB_n01_HFL29.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3537BGXB_n01_HFL29.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5529248
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG421590.7624725821666889No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATCA333150.6025231640903067No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA287650.5202334928728102No Hit
TCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCAT279090.5047521832987054No Hit
GATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACC240340.4346703204486397No Hit
CTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATC228100.4125334946090319No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT210160.38008785281470464No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG204710.37023117791063087No Hit
TTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATCAAT202270.36581828125633No Hit
CGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCAC198190.35843933930979405No Hit
TGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGC152620.2760230685981168No Hit
CTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGG148190.268011129180677No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG135400.24487959302964887No Hit
ATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCA127360.23033873684088685No Hit
GCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAACTG108830.19682604216703609No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATCAA94150.17027631967312734No Hit
CCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACT92920.16805178570395107No Hit
CGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAACTGT82500.1492065467130431No Hit
CCCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAC80860.14624050142080805No Hit
GCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCA70810.12806443118485553No Hit
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAACT61800.11176926771958864No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC58510.10581909149309274No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGGTA247800.068.95151
GTAGGTT181650.068.918888
AGTAGGT183100.068.908037
AGGTTGT191650.068.8948810
GGTAGTA240350.068.851594
TAGGTTG181600.068.84049
GGTTGTG56050.068.8196311
GAGGTAG253200.068.754662
GTTGTAT136700.068.753512
TAGTAGG182300.068.566216
GTGTGGT55900.068.5005615
TATAGTT149550.068.24023416
GTAGTAG242350.068.140455
TGTGTGG56650.068.0876714
TGTGGTT51450.067.9741916
TTGTATA166600.067.9462313
TGTATAG166250.067.9007514
AGGTAGT243500.067.890383
GTTGTGT56800.067.8481812
GTATAGT166100.067.56180615
Produced by FastQC (version 0.11.5)