FastQCFastQC Report
Fri 1 Mar 2019
H3537BGXB_n01_HFL28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3537BGXB_n01_HFL28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5393766
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATCA413130.7659397904914673No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG342540.6350664823056841No Hit
TCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCAT304680.5648743382638401No Hit
CTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATC258330.478941800589792No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA202480.37539633717888393No Hit
GATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACC165370.3065946872741606No Hit
TTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATCAAT155030.28742440810372566No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG142260.26374892792902027No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT140930.2612831183258599No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG138880.2574824343510638No Hit
CGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCAC131200.2432437743869497No Hit
ATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCA108340.20086151308751624No Hit
TGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGC94570.17533204072998348No Hit
CTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGG92330.1711790982404502No Hit
GCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAACTG91550.16973298433784484No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATCAA80480.14920929087394597No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC79300.14702158009820968No Hit
CGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAACTGT75590.14014326909992017No Hit
GAGCAGCTCCCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGG74040.13726958121653776No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC57580.1067528698871994No Hit
CCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACT56140.10408312114392801No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTTG163850.068.983889
AGTAGGT165100.068.885617
GTAGGTT164250.068.859088
TGAGGTA225250.068.7448041
AGGTTGT174550.068.6596610
TAGTAGG164750.068.655196
GGTAGTA218300.068.654854
GTTGTAT125400.068.480312
GAGGTAG230850.068.3000642
GGTTGTG50950.068.1861611
TATAGTT138600.068.0590116
GTTGTGT51050.068.0532212
GTAGTAG220000.067.870135
TTGTATA152700.067.81637613
CTGAGAC153100.067.7120744
TGTATAG153050.067.6390814
GTGTGGT50450.067.61368615
AGGTAGT221700.067.523743
GTATAGT152800.067.5210115
AGACCCT153400.067.395497
Produced by FastQC (version 0.11.5)