FastQCFastQC Report
Fri 1 Mar 2019
H3537BGXB_n01_HFL25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3537BGXB_n01_HFL25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5082691
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG427320.84073574411665No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA250900.4936361466789935No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATCA232950.4583202087240794No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC232850.4581234625516286No Hit
TCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCAT192230.378205167302124No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG178300.3507984254797311No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT172390.33917072668789033No Hit
CTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATC149940.29500121097269144No Hit
GATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACC149350.29384040855523186No Hit
TTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATCAAT138680.2728475919547342No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG138150.2718048372407451No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC135910.26739772297784775No Hit
CGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCAC120590.2372562093583891No Hit
GCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAACTG98660.1941097737399342No Hit
TGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGC91900.18080973248226184No Hit
CTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGG91020.17907836616469502No Hit
ATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCA90570.17819300838866656No Hit
CGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAACTGT73820.1452380245031618No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATCAA68280.1343382865493889No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC61720.12143173763661808No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT59660.11737876648413212No Hit
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAACT58680.11545065399411454No Hit
CCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACT56840.11183052442102029No Hit
CCCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAC52230.10276052587103958No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA51680.10167842192256031No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT207600.069.094038
AGTAGGT209000.069.065117
GGTAGTA280650.069.0361564
TGAGGTA290750.068.909791
AGGTTGT221400.068.9049210
TAGGTTG208200.068.860019
GTTGTAT158150.068.7622912
GGTTGTG64900.068.7377211
GTTGTGT64800.068.7346312
TAGTAGG209000.068.68816
GAGGTAG298250.068.5472262
TATAGTT176300.068.47700516
AGCTTAT47900.068.412172
GTAGTAG282850.068.364685
GCTACAT63600.068.222962
GTGTGGT64500.068.1315915
TGTATAG196150.067.9412914
TTGTATA196000.067.8863513
GGATAGG24500.067.8685314
CTGAGAC186700.067.662164
Produced by FastQC (version 0.11.5)