FastQCFastQC Report
Fri 1 Mar 2019
H3537BGXB_n01_HFL24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3537BGXB_n01_HFL24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4915704
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATCA307050.6246307751646559No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG300360.6110213308205701No Hit
TCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCAT270610.5505010065699643No Hit
CTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATC220870.44931509301617834No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA203450.4138776460096051No Hit
GATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACC178710.36354914779246267No Hit
TTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATCAAT155730.31680101161501995No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT148830.30276436498210635No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG147360.2997739489603117No Hit
CGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCAC145850.2967021610739784No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG133110.2707852222184249No Hit
ATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCA107040.21775110950537296No Hit
TGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGC106310.216266072977543No Hit
CTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGG102480.2084747169479692No Hit
GCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAACTG96710.19673682548827187No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC88820.18068622520802718No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATCAA81770.16634443408309368No Hit
CGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAACTGT79500.16172658077052646No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC70060.14252282073940986No Hit
CCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACT67610.13753879403641878No Hit
GAGCAGCTCCCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGG63510.12919817792120925No Hit
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAACT60050.12215951163861778No Hit
CCCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAC57840.11766371612285849No Hit
GCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCA52700.10720743152964457No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGGT156600.068.9422457
GTAGGTT155450.068.935328
GGTAGTA203400.068.9036944
AGGTTGT164550.068.8792510
TGAGGTA210050.068.801781
TAGGTTG155650.068.732419
GTTGTAT120150.068.6267912
GGTTGTG45750.068.5025611
TAGTAGG156650.068.4790346
GAGGTAG215750.068.429482
GTTGTGT45800.068.2752112
TATAGTT129900.068.1006416
GTAGTAG205500.068.028855
TTGTATA144950.067.7305713
TGTATAG145200.067.6620814
GTGTGGT45700.067.6587115
CTGAGAC128550.067.626844
GTATAGT144850.067.4878915
AGGTAGT207650.067.4281853
AGACCCT128950.067.305257
Produced by FastQC (version 0.11.5)