FastQCFastQC Report
Fri 1 Mar 2019
H3537BGXB_n01_HFL20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3537BGXB_n01_HFL20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4519473
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG538551.1916212354847566No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA307650.6807209601650458No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC283480.6272412734847626No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG253480.5608618526983123No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC216970.4800780976012027No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG214940.47558642346131946No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT200960.4446536133748337No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA92330.20429373070709791No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC75790.16769654338016843No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT69990.15486318869478807No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATCA52590.11636312463864704No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA50650.11207058876112326No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC46240.1023128139055151No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGGT353550.069.414937
GTAGGTT350350.069.412138
TAGTAGG351250.069.4003756
TAGGTTG350400.069.389959
AGGTTGT374400.069.28345510
GGTAGTA465850.069.2676854
TGAGGTA483800.069.140981
TATAGTT295650.069.1336716
GTTGTAT268200.069.1336612
GTAGTAG465450.069.0644155
GTTGTGT108000.068.9186712
AGCTTAT66100.068.8925252
TGTATAG323100.068.8850514
GGTTGTG108650.068.82811
GCTACAT90700.068.81822
GAGGTAG497750.068.794752
AGACCCT307250.068.786157
GTGTGGT107300.068.78207415
TGAGACC307000.068.759465
TCCCTGA307050.068.6390151
Produced by FastQC (version 0.11.5)