FastQCFastQC Report
Fri 1 Mar 2019
H3537BGXB_n01_HFL19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3537BGXB_n01_HFL19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4474927
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG499031.1151690295729964No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC330020.7374868908476049No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA264420.5908923207015444No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC224370.5013936540193841No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG208700.4663763230104089No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT175130.39135833947682275No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG168830.37727989752682No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA99530.22241703607678961No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC86410.19309812204757754No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT84780.18945560452718No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG47170.10540954075898892No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC44960.10047091270986097No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT384500.069.352388
GGTAGTA512800.069.324534
AGTAGGT389400.069.2065667
TAGGTTG385050.069.205629
TAGTAGG386600.069.1610646
TGAGGTA531400.069.159421
AGGTTGT409550.069.13618510
GTAGTAG512850.069.11495
TATAGTT324450.069.0984916
GAGGTAG543550.069.059252
GTTGTAT293600.069.0389112
GTGTGGT115900.068.9802115
TGTATAG355850.068.8913714
TTGTATA355600.068.84154513
CAGCACG96200.068.83674
GGTTGTG118600.068.824811
GTTGTGT118450.068.7924212
CTGAGAC318700.068.771734
TGAGACC318400.068.750255
GCTACAT92900.068.722522
Produced by FastQC (version 0.11.5)