FastQCFastQC Report
Fri 1 Mar 2019
H3537BGXB_n01_HFL18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3537BGXB_n01_HFL18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3904549
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG539201.3809533444195476No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC368500.943770970731831No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA271280.6947793458348198No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG214890.5503580567179461No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC209040.5353755324878751No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG186550.4777760504478238No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT174200.4461462770732292No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT112640.28848402209832685No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA101310.2594665862817959No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC92930.23800444046162567No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC47260.1210383068569507No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG44790.11471235218203177No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT366200.069.333778
TAGGTTG366100.069.276359
TAGTAGG366550.069.275546
AGTAGGT370600.069.255377
GGTAGTA490950.069.208614
TGAGGTA508050.069.195641
AGGTTGT389400.069.18671410
TATAGTT308450.069.15176416
GTTGTAT281850.069.1223112
GTTGTGT110100.069.08514412
GAGACCC330700.069.061486
AGACCCT330900.069.022347
TGAGACC330800.068.9877855
GAGGTAG520700.068.958692
TGTATAG337250.068.9564414
GTGTGGT108800.068.9463315
GTAGTAG491400.068.925685
TCCCTGA330450.068.913711
CTGAGAC331600.068.873154
CTAACTT287500.068.78474412
Produced by FastQC (version 0.11.5)