FastQCFastQC Report
Fri 1 Mar 2019
H3537BGXB_n01_HFL17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3537BGXB_n01_HFL17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2983694
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG458331.5361159689968207No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA268070.8984500421289852No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG248320.8322569271513768No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC215140.7210524939889948No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT175850.5893700895601224No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG127850.42849568353859346No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC119750.40134812752246046No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT62670.21004164636185882No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA59020.1978084884039717No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC52260.1751520095559397No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC52080.17454873053335898No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA45810.15353451124679673No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTAACTGTAGGCACCATCA36920.12373923063155941No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT36770.12323649811274212No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC35180.11790753341327898No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC32310.10828858455324171No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATCA30190.10118329828729085No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT283150.069.4700558
AGTAGGT286150.069.4388667
GGTAGTA372850.069.379454
TAGGTTG283450.069.371889
TAGTAGG283850.069.3514566
TATAGTT242250.069.3124516
AGGTTGT304850.069.30652610
GTTGTAT222400.069.2511812
GTTGTGT83950.069.2136812
GTAGTAG372650.069.201045
GAGGTAG398650.069.1062
TGTATAG265300.069.0990914
AGACCCT239250.069.0606167
TGAGGTA390500.069.035371
TTGTATA265600.069.007913
TGAGACC239400.069.000455
GAGACCC239750.068.9917766
CTGAGAC239750.068.9288564
GCTACAT62050.068.920042
GGTTGTG84350.068.8854611
Produced by FastQC (version 0.11.5)