FastQCFastQC Report
Fri 1 Mar 2019
H3537BGXB_n01_HFL16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3537BGXB_n01_HFL16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4125172
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG562981.3647430943485508No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA298220.7229274318743557No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG237260.5751517754896038No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT191130.46332613524963323No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC190030.46065957977024957No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG157910.38279615977224707No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC113100.27417038610753686No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA53780.1303703215284114No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATCA48990.11875868448636807No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC47740.11572850780525032No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT45490.1102741897792383No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT369850.069.484148
TAGTAGG370750.069.427546
TAGGTTG370050.069.426869
AGGTTGT395050.069.3405610
AGTAGGT374200.069.338527
TGAGGTA502200.069.292421
GAGGTAG513100.069.256862
GTTGTGT113700.069.2347412
TATAGTT306650.069.22977416
GGTAGTA484650.069.225214
GTTGTAT284050.069.15433512
CTGAGAC334100.069.062264
TGTATAG336750.069.0595614
GTAGTAG484200.069.05865
GTGTGGT112050.068.94292415
GGTTGTG114500.068.93410511
TTGTATA337200.068.9155913
TGAGACC334000.068.905085
CAGCACG92900.068.872944
AGACCCT334900.068.857647
Produced by FastQC (version 0.11.5)