FastQCFastQC Report
Fri 1 Mar 2019
H3537BGXB_n01_HFL15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3537BGXB_n01_HFL15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4507929
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG632631.4033717035028723No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA353540.7842625737894274No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG290700.6448637500723725No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC236060.5236550974959899No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT234080.5192628366595836No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG157730.34989459683149404No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC141550.31400228353197224No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA69370.15388441122298066No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT68070.15100060360311798No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC60240.13363120847732962No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC50370.11173645370191057No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA46830.1038836237216691No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT397700.069.410198
TAGTAGG398350.069.407566
AGTAGGT401700.069.371337
AGGTTGT425000.069.3563410
TAGGTTG398000.069.329229
GTTGTAT303150.069.3210512
GGTAGTA539150.069.270494
TGAGGTA558950.069.2402041
TATAGTT337950.069.22294616
GCTACAT95600.069.2225652
TGTATAG370500.069.2047314
GAGGTAG571600.069.1835862
GTAGTAG538650.069.081815
CTGAGAC364650.068.8927464
TTGTATA371950.068.8684213
TAGTTAA332450.068.84283418
GTTGTGT124700.068.83761612
TGAGACC364700.068.8353965
TCCCTGA364750.068.814021
GAGACCC365500.068.7627266
Produced by FastQC (version 0.11.5)