FastQCFastQC Report
Fri 1 Mar 2019
H3537BGXB_n01_HFL14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3537BGXB_n01_HFL14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4678817
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG608981.3015683237878293No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA337480.7212934380635105No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG286230.6117572027288094No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT225400.48174570623300716No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC171740.3670585962220792No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG162220.34671157260478447No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC112930.24136443036776178No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA55600.11883345726067081No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA49070.10487693790973232No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC47350.10120079498727991No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT391150.069.428898
AGGTTGT417500.069.373210
TAGGTTG391550.069.3572169
TATAGTT325500.069.3547616
AGTAGGT395550.069.290397
GTTGTGT119400.069.23991412
GGTAGTA515550.069.193894
TAGTAGG392800.069.193576
TGAGGTA535250.069.1921
GGTTGTG119900.069.0677411
GTTGTAT301300.068.9559712
TGTATAG359350.068.9487914
CAGCACG100700.068.948334
GAGGTAG548850.068.9373552
GTAGTAG516500.068.86445
TTGTATA359700.068.8525613
CTGAGAC348400.068.8421554
TGAGACC348150.068.8421555
GTGTGGT118650.068.7035515
GACCCTA299300.068.681998
Produced by FastQC (version 0.11.5)