FastQCFastQC Report
Fri 1 Mar 2019
H3537BGXB_n01_HFL13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3537BGXB_n01_HFL13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4939557
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG759701.537992172172525No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA444340.8995543527486373No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG406700.8233531873404842No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT301390.6101559309873335No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC230300.4662361422289488No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG171380.3469541904263884No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC126570.25623755328666115No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC81870.1657436081818673No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT69560.14082234499976415No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA66660.1349513731696992No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA64570.1307202245059628No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTAACTGTAGGCACCATCA60200.1218732772999684No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC59210.11986904898556694No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT55950.11326926685935601No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGGT478400.069.5021747
GTAGGTT473500.069.4761058
GTTGTGT155250.069.4079312
TAGTAGG474650.069.367036
AGGTTGT507000.069.33681510
TAGGTTG473950.069.335729
GGTAGTA610950.069.327814
GTTGTAT353350.069.2674712
TATAGTT376150.069.2103516
GTAGTAG611400.069.156755
TGAGGTA638200.069.138571
TGAGACC437600.069.089795
CTGAGAC438050.069.0826344
GAGGTAG652300.069.064742
AGACCCT439000.069.046257
TGTATAG414150.069.0439314
GGTTGTG156250.069.00844611
GAGACCC440050.068.936816
GTGTGGT154750.068.9082915
TTGTATA415200.068.88615413
Produced by FastQC (version 0.11.5)