FastQCFastQC Report
Fri 1 Mar 2019
H3537BGXB_n01_HFL12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3537BGXB_n01_HFL12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4892058
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG643741.3158879146567763No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA404060.825950959698352No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG373500.7634823626375649No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC350690.7168557690853216No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT274830.5617881063552395No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC220190.45009687129629294No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG170950.34944393545620267No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT100630.20570075007287325No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA98670.20169425628232535No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC91350.18673122845232007No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC90210.1844009208394504No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA75640.15461795424338798No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT67400.13777432728720715No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC57790.11813024293661277No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATCA52220.10674444170531093No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC50190.10259485885081494No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG49300.1007755836091886No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT416500.069.46278
AGTAGGT421950.069.4274147
TAGGTTG416800.069.387559
GGTAGTA551350.069.3188864
AGGTTGT445450.069.3109710
GTTGTGT132300.069.30112512
TAGTAGG418050.069.298656
GTAGTAG550800.069.148425
GAGGTAG586750.069.13962
GTTGTAT315650.069.11835512
TATAGTT346600.069.0440216
GGTTGTG133150.069.0438211
TGAGGTA576100.069.0434951
TGTATAG380250.069.01768514
TGAGACC349750.069.001485
GCTACAT104850.068.9234852
AGACCCT350900.068.887117
CAGCACG98200.068.857334
GTGTGGT131700.068.84675615
TGTGGTT121500.068.8454716
Produced by FastQC (version 0.11.5)