FastQCFastQC Report
Fri 1 Mar 2019
H3537BGXB_n01_HFL11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3537BGXB_n01_HFL11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4483864
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG617801.3778294792170325No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA370720.8267868963019396No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG325590.7261370996087303No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC301200.6717420510523959No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT241800.5392670250480389No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC199760.44550860597020786No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG147390.3287120215956595No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA91980.20513557057038304No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC75170.1676455842550086No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT74610.16639666145092713No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC70020.156159954896045No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA65550.14619087465632322No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTAACTGTAGGCACCATCA54000.12043184182214269No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT52590.11728723261900896No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC50740.11316132692695408No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATCA48280.10767498746616756No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC46290.10323685107309231No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT404100.069.487088
TAGTAGG405000.069.4385456
AGTAGGT409100.069.41447
TAGGTTG404350.069.4087759
GGTAGTA543650.069.367984
AGGTTGT434350.069.3215210
GTTGTAT318150.069.265912
GTAGTAG543300.069.187535
TATAGTT347150.069.15559416
GAGGTAG579500.069.142052
TGAGACC345700.069.116695
TGTATAG382000.069.1044814
TGAGGTA568450.069.052121
AGACCCT346650.069.025797
TTGTATA382300.068.9771213
CAGCACG103750.068.918264
CTGAGAC348350.068.861784
TGTGGTT108600.068.8607316
GTTGTGT119300.068.8374512
GAGACCC347750.068.830616
Produced by FastQC (version 0.11.5)