FastQCFastQC Report
Fri 1 Mar 2019
H3537BGXB_n01_HFL10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3537BGXB_n01_HFL10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4557892
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG634151.39132300633714No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC375080.8229242816635409No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA328430.7205743356797397No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG253450.5560684632281766No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT216150.47423238637510495No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC209630.459927527901056No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG175000.3839494222329094No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT118290.25952786946246204No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA103550.2271883581269587No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC91170.20002667899985346No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATCA52360.1148776671320865No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA51570.11314440974029222No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC49850.10937073541891734No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC47110.10335918446509922No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG45730.1003314690212054No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTTGT400650.069.3403610
GTAGGTT374200.069.3114858
TAGGTTG373950.069.310349
TAGTAGG374550.069.293156
AGTAGGT378100.069.280397
GGTAGTA499300.069.270564
GAGGTAG532400.069.172552
TGAGGTA520900.069.098121
GTTGTAT288700.069.09514612
GTAGTAG499000.069.067125
TATAGTT316850.069.0304316
TGTATAG348400.068.89596614
GTTGTGT116150.068.7988512
TGTGGTT105150.068.7497616
CTGAGAC355250.068.735594
TTGGCGA88200.068.72996517
AGACCCT354750.068.728697
GTGTGGT114400.068.7174415
TTGTATA349100.068.6877613
TGAGACC355200.068.686255
Produced by FastQC (version 0.11.5)