FastQCFastQC Report
Fri 1 Mar 2019
H3537BGXB_n01_HFL09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3537BGXB_n01_HFL09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4281002
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG627121.464890696150107No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC391500.9145055293129972No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA372790.8708008078482561No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG330090.7710578037571578No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT255890.5977338950086919No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC228360.5334265202398878No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG143890.3361129006713849No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA105990.24758222490902831No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC95890.2239896173839676No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT89150.20824563968902607No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC74390.17376773007814528No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA69660.16271891487086435No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT62020.14487262561428377No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATCA51440.12015878525634886No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC50250.11737906219151496No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG50170.11719219005270262No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC44540.10404106328378263No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG44210.10327021571118163No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG42910.10023354345548076No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT375700.069.467938
AGTAGGT379850.069.426417
TAGGTTG376050.069.345919
GGTAGTA480600.069.327044
AGGTTGT402850.069.32204410
GTTGTAT283000.069.3100112
TAGTAGG376650.069.280636
TATAGTT301550.069.21426416
TGAGGTA502050.069.153981
GGTTGTG121350.069.1005511
GAGGTAG513300.069.049122
GTAGTAG481050.069.044295
TGTATAG330450.068.9963214
GTTGTGT121400.068.9857512
TGTGGTT109600.068.982116
AGACCCT343350.068.930767
GTGTGGT120150.068.85692615
TGAGACC343850.068.832945
GAGACCC344400.068.818346
TCCCTGA343100.068.791791
Produced by FastQC (version 0.11.5)