FastQCFastQC Report
Fri 1 Mar 2019
H3537BGXB_n01_HFL08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3537BGXB_n01_HFL08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4589008
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG753931.6429040873321643No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA450250.981148867031829No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG421400.9182812494552199No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC329940.7189789165763059No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT306680.6682925808802251No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG187430.4084324978295963No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC160910.3506422303033684No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC110290.24033516611869057No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA89630.19531454292518124No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA86000.18740433662351427No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC69950.1524294575211026No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT68850.15003242530847624No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT64910.14144669174688734No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC63470.13830875866854012No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC54850.11952474260232278No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA54830.11948116019845684No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG52120.11357574447462283No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATCA49800.10852018562617453No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT439250.069.635628
TAGGTTG439200.069.592799
AGTAGGT444700.069.574187
AGGTTGT471200.069.5227310
TAGTAGG440750.069.4703756
GGTAGTA574700.069.397764
GTTGTGT141450.069.3510112
GTTGTAT331500.069.2795212
GTAGTAG575750.069.224165
TATAGTT362600.069.20186616
TGTATAG395500.069.1872614
GAGGTAG612300.069.182652
TGAGGTA601700.069.156951
AGACCCT383600.069.062147
GGTTGTG142450.068.988311
TGAGACC384000.068.969685
TAGTTAA356150.068.9348118
TTGTATA396800.068.8989613
CTGAGAC385550.068.817784
GACCCTA323850.068.795438
Produced by FastQC (version 0.11.5)