FastQCFastQC Report
Fri 1 Mar 2019
H3537BGXB_n01_HFL07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3537BGXB_n01_HFL07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4904122
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG624251.2729087897894873No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA408230.8324221950432719No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG385760.7866035959138047No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT274930.5606100337634341No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC232390.4738666778681281No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG158740.3236868903342943No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC157470.32109723208354113No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC122430.24964713357457255No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA94840.1933883374026992No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATCA93700.1910637622799759No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA77040.15709233987245833No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC71300.14538790021944803No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT70710.1441848306383895No Hit
CTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATC65090.13272508310356063No Hit
TCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCAT63130.12872844517326446No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG62720.127892413769478No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA61980.12638347904069272No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT60900.1241812499770601No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC56820.11586171795889254No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGGT392600.069.381757
GTAGGTT388300.069.3763358
GGTAGTA520350.069.315334
AGGTTGT417000.069.293110
TAGTAGG389200.069.255346
TAGGTTG388750.069.2324149
TATAGTT332300.069.22562416
GTTGTGT119250.069.1359512
GAGGTAG555150.069.1181262
GTAGTAG520950.069.042425
GTTGTAT300650.069.0200912
TGTATAG364450.068.8994914
CAGCACG106400.068.887544
AGACCCT344850.068.797327
TGAGGTA547300.068.768051
TCCCTGA343400.068.7520751
CTGAGAC344650.068.7372744
TTGTATA365050.068.6809813
TGAGACC344700.068.657765
GACCCTA293750.068.5986258
Produced by FastQC (version 0.11.5)