FastQCFastQC Report
Fri 1 Mar 2019
H3537BGXB_n01_HFL06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3537BGXB_n01_HFL06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4133086
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG592641.4338922538751915No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA366510.8867708051562441No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG337860.8174521410877973No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT247330.5984148406299796No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG146350.3540937691594126No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC135270.327285713387043No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC90320.2185292055379443No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC90260.2183840355608376No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA75360.18233349124600842No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC51770.125257495246893No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT50060.12112015089935221No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA49960.12087820093750772No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA48990.11853128630761615No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG42970.10396589860457779No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT305500.069.29758
AGTAGGT309900.069.260697
GGTAGTA398950.069.248994
TAGTAGG306650.069.1738366
TAGGTTG305500.069.171699
AGGTTGT327600.069.1350410
GTTGTAT232550.069.01018512
GAGGTAG425350.068.993772
TATAGTT252700.068.97871416
GTAGTAG399600.068.952715
TGAGGTA418650.068.893431
TGTATAG276100.068.87855514
TGAGACC300550.068.876875
GAGACCC300900.068.846546
TGTGGTT87950.068.80482516
GTTGTGT97200.068.80321512
AGACCCT300850.068.765867
TCCCTGA300750.068.6949841
CAGCACG72200.068.678434
TTGTATA277000.068.5916913
Produced by FastQC (version 0.11.5)