FastQCFastQC Report
Fri 1 Mar 2019
H3537BGXB_n01_HFL05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3537BGXB_n01_HFL05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4438434
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG790961.7820699823406183No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA402550.9069640328097702No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC330290.7441588632387008No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG316150.7123007799597786No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT260860.5877298164172319No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG200920.4526821847525502No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC158860.3579190318026583No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT107740.24274327386641323No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA90830.20464425065236974No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC86400.19466325284999167No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC52450.11817231032386648No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC48380.10900240940836341No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA47310.10659164921681837No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT426300.069.452968
TAGGTTG426350.069.411259
AGTAGGT431850.069.3913657
GTTGTAT319000.069.3189912
TAGTAGG427550.069.277546
AGGTTGT459350.069.2622610
TATAGTT344300.069.22655516
GGTAGTA554250.069.22514
TGAGGTA579450.069.178751
GAGGTAG592050.069.0965962
GTAGTAG553800.069.067625
GTTGTGT142200.069.0421912
TGAGACC448750.069.001665
AGACCCT448850.068.99727
CAGCACG117700.068.990144
GACCCTA390200.068.965818
GAGACCC450000.068.95836
TGTATAG376400.068.9122114
CTGAGAC449750.068.894084
GGTTGTG142850.068.8755611
Produced by FastQC (version 0.11.5)