FastQCFastQC Report
Fri 1 Mar 2019
H3537BGXB_n01_HFL04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3537BGXB_n01_HFL04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4245926
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG635171.4959516487098456No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA373410.8794547997303769No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG336800.7932309701111137No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC278130.6550514540291094No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT251950.593392348335793No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC161480.38031750906633793No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG147090.3464261977245953No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA84780.19967375785635452No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC81900.19289078519032127No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT75980.17894800804347508No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA70210.1653585107229848No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC68660.16170795251730716No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT55940.1317498232423269No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC53230.12536723437949696No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC47700.11234298478117612No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA45750.10775034703854942No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT380900.069.546898
GGTAGTA504500.069.3748254
AGTAGGT385850.069.360977
GTTGTAT293350.069.3300612
TAGGTTG382050.069.317639
TAGTAGG383000.069.3156056
TATAGTT324150.069.3058516
AGGTTGT408100.069.2529510
GTTGTGT116050.069.1854712
GAGGTAG535800.069.170412
GTAGTAG504500.069.167055
TGTATAG352950.069.1148914
TGAGGTA526250.069.0286561
TTGTATA353500.068.90851613
TGAGACC324800.068.804665
GTGTGGT114750.068.780215
GTATAGT354150.068.76150515
AGACCCT325550.068.750237
GAGACCC325750.068.712076
CAGCACG85750.068.6987464
Produced by FastQC (version 0.11.5)