FastQCFastQC Report
Fri 1 Mar 2019
H3537BGXB_n01_HFL03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3537BGXB_n01_HFL03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3385430
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG510381.5075780624617847No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG354671.0476364893085959No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA331820.9801413705201408No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC254300.7511601185078409No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT232640.687180062798522No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG177320.5237739371364937No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC138780.40993315472480596No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC131660.3889018529403946No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA110460.3262805611104055No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC86800.2563928363605214No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT79980.23624768493219475No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT78070.23060586099845512No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA77120.22779971820418676No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA74360.21964713492820703No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG67530.19947244515467755No Hit
CTGCAGTGATGACTTTCTTAGGACACCTTTGGATTTACCGTGAAAATTAA58080.17155870893800787No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC57500.16984548491624402No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC53150.15699630475301513No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCC44080.13020502565405281No Hit
GCTTAATGATGACTGTTTTTTTTGATTGCTTGAAGCAATGTGAAAAACAC42690.12609919567086011No Hit
CATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCT42310.12497673855315276No Hit
TTGCTGTGATGACTATCTTAGGACACCTTTGGAATAACTATGAAAGAAAA37210.109912182499712No Hit
GTGAAATGATGGCAATCATCTTTCGGGACTGACCTGAAATGAAGAGAATA36730.10849434192997641No Hit
CCACAATGATGACAGTTTATTTGCTACTCTTGAGTGCTAGAATGATGAGG35000.10338420820988767No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTTG299500.069.4667749
GTAGGTT299550.069.45628
AGTAGGT303950.069.416467
TAGTAGG300350.069.403286
GTTGTGT98400.069.3541212
AGGTTGT323250.069.3354810
GGTAGTA396950.069.249294
TATAGTT246900.069.2367816
GTTGTAT225950.069.2217912
GTAGTAG396700.069.142155
GGTTGTG98950.069.03924611
GAGGTAG424050.069.017392
TGTATAG272450.068.98797614
AGACCCT245200.068.904497
CAGCACG69300.068.7825554
GACCCTA203300.068.7362068
TGAGGTA416850.068.728431
GAGACCC246250.068.643126
TGAGACC245750.068.625345
TCCCTGA245500.068.620981
Produced by FastQC (version 0.11.5)