FastQCFastQC Report
Fri 1 Mar 2019
H3537BGXB_n01_HFL02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3537BGXB_n01_HFL02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4545083
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG608211.338171382128775No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG407490.8965512841019626No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA403460.8876845593358801No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT276610.6085917462893417No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC231580.5095176479725453No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG192310.42311658554970283No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC157410.3463303090394609No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC133490.2937020072020687No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA114830.2526466513372803No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA85650.18844540352728434No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC77050.16952385688006139No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT70850.15588274185531925No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG70100.154232606973294No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA63400.1394914020272017No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT60110.1322528103447176No Hit
CTGCAGTGATGACTTTCTTAGGACACCTTTGGATTTACCGTGAAAATTAA55030.12107589674379983No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATCA54380.11964577984604462No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC52780.11612549209772406No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC48300.10626868640242652No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT378650.069.490458
TAGTAGG379100.069.388716
GGTAGTA486050.069.330594
AGGTTGT407050.069.3208410
TAGGTTG379600.069.2981349
AGTAGGT385200.069.233237
GTAGTAG486850.069.051595
GAGGTAG518950.069.026852
GTTGTAT285700.069.0234712
GTTGTGT125050.069.01337412
GTGTGGT123250.068.9999615
AGACCCT351950.068.9937067
TATAGTT305700.068.97584516
TGAGGTA511350.068.907711
TGTATAG334700.068.8870614
TGAGACC353750.068.787735
GAGACCC354350.068.6636666
TCCCTGA353000.068.638271
TTGTATA335400.068.6286913
GGTTGTG126150.068.5216211
Produced by FastQC (version 0.11.5)