FastQCFastQC Report
Fri 1 Mar 2019
H3537BGXB_n01_HFL01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3537BGXB_n01_HFL01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4076738
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG513291.2590703645905132No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA318600.7815071755898956No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC314400.7712048211094262No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG280570.6882218087107879No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT214040.5250276078570661No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC177480.43534806504612267No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG135130.3314659907013892No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA85080.2086962664757951No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC67430.1654018482423938No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC66850.16397914214747183No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA60700.14889355165821302No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT55390.13586843206504806No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT45520.11165789903594492No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC45170.1107993694959058No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATCA41290.1012819563091864No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG41220.10111025040117859No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT347100.069.521248
TAGTAGG348150.069.379456
AGTAGGT351700.069.367027
TAGGTTG348150.069.2998359
TATAGTT290500.069.2830816
GGTAGTA452800.069.279114
AGGTTGT374750.069.2767410
GTTGTAT266700.069.2006712
TGAGGTA473600.069.198281
GTAGTAG452050.069.1475455
GAGGTAG483150.069.064272
TGTATAG318350.069.0376514
TGAGACC301300.068.961815
GTTGTGT110350.068.9003612
GTGTGGT108300.068.8826215
TAGTTAA283250.068.8474318
GAGACCC302300.068.770056
AGACCCT302050.068.7615367
TAACTTG257700.068.74313
GACCCTA257350.068.729558
Produced by FastQC (version 0.11.5)