FastQCFastQC Report
Fri 4 Sep 2020
H33NHAFX2_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH33NHAFX2_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135828795
Sequences flagged as poor quality0
Sequence length91
%GC38

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3019590.2223085318543833No Hit
CTATAATTATTACCTGTATTAATTCATTATCTAATATAAAATCTAATCTA1392700.10253348710043404No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATG26216300.084.573228
GCTCGAT26234550.084.4935767
GGCTCGA8473900.084.258586
TCGATGA26362100.084.1501169
CGCTCGA5394500.083.56776
TGCTCGA7301150.083.334416
AGCTCGA5438350.082.892326
AGGCTCG1766150.082.091655
TTGCTCG2074550.081.896845
GAGCTCG1589450.080.88265
TGGCTCG2203550.079.659425
TCGCTCG1513000.079.5239265
CGGCTCG1899250.079.426115
CCGCTCG1153900.079.386235
GGGCTCG3077250.079.221315
ATGCTCG1756950.078.8284765
CTGCTCG1545950.078.307225
AAGCTCG1247150.077.637825
CAGCTCG1304200.077.2391055
TCGGCTC504550.076.8232964
Produced by FastQC (version 0.11.5)