FastQCFastQC Report
Fri 4 Sep 2020
H33NHAFX2_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH33NHAFX2_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135828795
Sequences flagged as poor quality0
Sequence length8
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGATATC3509720525.8392964466776No Hit
CGTCTGCG3409197725.09922656679683No Hit
AGATCTCG3380859424.89059407469528Illumina Single End PCR Primer 1 (100% over 8bp)
CTAGCGCT3035872022.350724675132398No Hit
GGGGGGGG1735400.12776377792352497No Hit
AGGGAAAG1412580.10399709428328507Illumina Single End PCR Primer 1 (100% over 8bp)

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)