FastQCFastQC Report
Fri 4 Sep 2020
H33NHAFX2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH33NHAFX2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135828795
Sequences flagged as poor quality0
Sequence length8
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGAGAGG3457309825.453437910569697No Hit
GCTACGCT3341491024.60075567923576No Hit
CTCTCTAC2991268822.022346587113578No Hit
GGGGGGGG80489765.925824491044039No Hit
CACCTGCC11831690.8710737660596931No Hit
CGTCCTAG11723170.8630842966692004No Hit
GAATCCGC11144130.8204541606954548No Hit
ATGGAATG10918420.8038369183794938No Hit
ACCGGCTC10775500.793314849034772No Hit
GACAGCAT10281030.7569109333554789No Hit
GTCTCCTT10192680.7504064215544282No Hit
GAGTTAGT9856670.7256686625247614No Hit
TGGAGGCT9554050.7033891451367142No Hit
TGATGCAT9139020.6728337684214898No Hit
GCTACTGA8622660.6348182651550431No Hit
AGGCCGTG8277810.6094296868348128No Hit
TGCACCGT8229070.6058413460857103No Hit
AGTGGAAC8228020.605764042889433No Hit
ATGACCGC8144680.5996283777677627No Hit
CAGATGGG8007390.5895208007992708No Hit
TCTCGTTT7951070.5853744045951376No Hit
TCACATCA7885090.5805168189852528Illumina PCR Primer Index 1 (100% over 8bp)
CCATATGC7728900.5690177844837687No Hit
TTAAAGCA7444940.5481120553267075No Hit
GGCTAGCG7254000.5340546531389018No Hit
CCCGATTA6986940.5143931373314473No Hit
TTTGTACA6873690.5060554354472481No Hit
AAGTTGCA6742410.49639032724982945No Hit
ATTCTAAG6669450.49101885943992946No Hit
GCACAATG6511220.4793696358713924No Hit
ACTGTGGC6097650.4489217474100392No Hit
CTTGGTAC5621260.4138489191485502No Hit
GGGTCAAG5105580.37588347890445467No Hit
CTAAGTTT4643070.34183252527566044No Hit
TACCACCA4119400.30327884451894016No Hit
CAAGTAAA3862450.28436164805849895No Hit
GCTAGCTA1513270.11141010269582381No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)