FastQCFastQC Report
Fri 4 Sep 2020
H33NHAFX2_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH33NHAFX2_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135828795
Sequences flagged as poor quality0
Sequence length28
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACCTGTCTCTTATACACATCT1887070.13893004057055797No Hit
CCCATGTACTCTGCGTTGATACCTGTCT1374310.10117957683420514No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTAGC11919300.021.8302656
TACTAGG10572050.021.826066
ATTCTAG11932100.021.8255565
TACACCG8909500.021.8009110
TGTACAC9125450.021.794968
GTACTAG10613100.021.7924525
TAGCATC11886200.021.7845829
GTACACC9123450.021.7787599
CAATCCA14104150.021.7735828
ATGTACA9146700.021.761527
ATAGCGG6424150.021.75562510
CTTCAAT14207200.021.747215
TGTGCTA7223800.021.7466876
GTCAATG12221300.021.7462968
AATAGCG6439700.021.7418359
TTCAATC14216900.021.7381736
GCTAGTG7207850.021.7332429
GATCCGT9691000.021.730585
AATCCAA14120900.021.7197789
CGTTATC9662950.021.7189319
Produced by FastQC (version 0.11.5)