FastQCFastQC Report
Fri 21 Feb 2020
H33NFAFX2_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH33NFAFX2_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13257028
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4542513.4264919709002655No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN524600.39571463528627987No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGCA4000.031.6506121
GATCCCG3500.029.1386591
GATCCAC7400.028.5140651
GATCAAC12100.026.738811
GATCCTA5000.026.0238361
GATCCAG6450.025.6258181
GATCATG9750.025.6090431
GATCCAT10050.024.8445951
GATCCGG3900.024.3466241
CGCCGTA4350.024.14496647
GATCCGA5750.023.8526361
GATCTTC14050.023.278031
GATCGTG6200.023.2558291
GATCGTC8250.022.1660861
GATCACG5700.021.5939871
GCCGTAT4950.021.21886848
GATCCAA10950.020.5544321
GATCGCG2402.1827873E-1120.5142861
GATCACA10650.020.4730111
GATCCTC8100.020.4057461
Produced by FastQC (version 0.11.5)