FastQCFastQC Report
Fri 21 Feb 2020
H33NFAFX2_n02_JK06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH33NFAFX2_n02_JK06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22808582
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCAAC243300.034.893161
GATCACG90300.034.4409451
GATCGTG110700.030.7516541
GATCATG171350.029.9639931
GATCCAC149800.029.6687761
GATCGCG37250.029.4284821
GATCCGG75700.029.1933061
GATCCTG112000.028.6123071
GATCCGA123400.026.6501871
GATCGCA81750.026.4330461
GATCCAG143650.026.3797951
GATCACA230250.026.2537251
GATCCCA103350.025.9578111
GATCCAA239000.025.6442471
GATCCTA87000.025.4417881
GATCGTT160700.025.4334221
GATCATA190900.025.3176231
GATCCAT223300.025.1416991
GATCCCG61300.024.3959221
GATCGTA104850.023.9163231
Produced by FastQC (version 0.11.5)