FastQCFastQC Report
Fri 21 Feb 2020
H33NFAFX2_n02_DJ61.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH33NFAFX2_n02_DJ61.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26280866
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCACG64500.020.8515511
GATCCGG49900.020.7056261
GATCAAC158550.019.9916761
GATCCGC40150.019.9763811
GATCCAC95450.019.7044931
GATCGCG23600.019.1445261
GATCGTG74150.019.1298141
GATCCCG41600.018.4381641
GATCCTG73900.018.4362281
GATCCTT149800.018.1433221
GATCCAG95100.017.4199731
GATCCTC105650.017.3048531
GATCATG110800.017.1011141
GATCCGA88700.017.018471
GATCGCA61400.016.4852811
GATCGTC93150.016.1302591
GATCTGC69900.016.0840241
GATCACA149900.015.8648161
GATCCCA75550.015.7156421
GATCCAT141150.015.5579921
Produced by FastQC (version 0.11.5)