FastQCFastQC Report
Fri 21 Feb 2020
H33NFAFX2_n02_DJ60.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH33NFAFX2_n02_DJ60.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27766219
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCACG88600.032.334841
GATCAAC224650.032.1308861
GATCCAC140050.031.0591531
GATCGTG100600.028.47781
GATCGCG35650.027.9003681
GATCCGG68050.026.7624781
GATCCTG101900.026.636591
GATCATG151500.026.4462741
GATCACA204250.026.3549941
GATCCCG59800.025.59361
GATCCAG137650.025.5706041
GATCCGC58000.025.5425051
GATCGCA75500.025.374151
GATCCGA114200.025.1170651
GATCCAA235350.024.6580891
GATCACC97850.024.4103871
GATCCCA103100.024.3563181
GATCCAT195800.024.2011621
GATCGAC94900.023.4715651
GATCGCC54250.023.3055081
Produced by FastQC (version 0.11.5)