FastQCFastQC Report
Fri 21 Feb 2020
H33NFAFX2_n02_DJ59.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH33NFAFX2_n02_DJ59.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23048288
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCGC44200.043.025491
GATCGCG29000.042.0271071
GATCTGC73550.041.808071
GATCCCG49500.041.319581
GATCCGG45550.041.288981
GATCCAG85150.040.7191621
GATCACG59700.039.5397451
GATCCTG74550.039.4150961
GATCAGC80350.038.7493251
GATCCGT59550.037.933791
GATCCGA79150.037.6111221
GATCTCG76000.037.511041
GATCTGG71550.036.6134151
GATCGTG71450.036.5176051
GATCCAC93550.036.3516161
GATCGCC39700.035.2872431
GATCTTG108150.034.9740141
GATCGGC53900.034.827661
GATCGGG38600.034.7503931
GATCTCC84800.034.444391
Produced by FastQC (version 0.11.5)