FastQCFastQC Report
Fri 21 Feb 2020
H33NFAFX2_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH33NFAFX2_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13257028
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5989104.517679226445022No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCAC7150.034.726551
GATCGTG5650.032.185791
GATCATG8650.029.5131841
GATCGCG1900.029.4493481
GATCCGC3550.028.567941
GATCCAG7550.027.328411
GATCCCA6450.026.0250051
GATCCCG3450.025.3413771
GATCCAA13700.025.2710881
GATCAAC11350.025.2654671
GGATCGT1551.2478267E-924.91648365
GATCCTT13050.024.6539571
GATCTTG8950.024.6165281
AGTGGCG5150.024.41482420
CCGATCT3200.024.1250335
GATCGTC7400.023.629121
GATCGAC5100.023.3140661
GATCCTA4800.023.3140661
GATCTGC4700.023.0660461
GATCCTC9450.022.9441
Produced by FastQC (version 0.11.5)