FastQCFastQC Report
Fri 21 Feb 2020
H33NFAFX2_n01_JK06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH33NFAFX2_n01_JK06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22808582
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCAAC238700.034.142011
GATCGTG106950.032.451741
GATCGCG36600.031.7688981
GATCACG86600.031.220841
GATCATG156300.030.3168451
GATCCTG104250.029.395251
GATCCAC152650.028.0132851
GATCCGA113900.027.5504971
GATCCGG67050.027.2657151
GATCCAA231400.026.7737771
GATCGCA78400.026.5347821
GATCACA219800.026.0357171
GATCCCA106950.025.2147731
GATCCAG132700.025.1780821
GATCCTA87650.024.1740061
GATCCAT216600.024.059671
GATCCCG59050.023.8424741
GATCGTT157300.023.8232061
GATCGTA98800.023.5018331
GATCATA188850.022.88461
Produced by FastQC (version 0.11.5)