FastQCFastQC Report
Fri 21 Feb 2020
H33NFAFX2_n01_DJ61.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH33NFAFX2_n01_DJ61.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26280866
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGTG67150.019.451821
GATCCGG45800.019.1913571
GATCAAC160900.018.9565621
GATCCAC94300.018.8646811
GATCACG57300.018.3954051
GATCCTG73650.018.3532091
GATCCGC39750.018.3241421
GATCCCG46650.018.3162121
GATCCAG95350.018.289681
GATCGCG25050.017.6140921
GATCCCA72350.017.1341341
GATCATG105900.016.9305651
GATCCTT156300.016.8707141
GATCCGA81850.016.6428761
GATCGTC89550.016.4240841
GATCGCC40600.016.3879641
GATCCGT63100.016.1495741
GATCTGC69800.015.4522871
GATCGAC71250.015.4327121
GATCCCC38800.015.2529011
Produced by FastQC (version 0.11.5)