FastQCFastQC Report
Fri 21 Feb 2020
H33NFAFX2_n01_DJ60.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH33NFAFX2_n01_DJ60.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27766219
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCAAC215300.031.5202561
GATCACG79700.031.01891
GATCCAC137700.029.7530781
GATCGCG33800.028.2830311
GATCCGC56900.027.7551961
GATCATG145500.026.8103331
GATCCGA117750.026.4970051
GATCCGG62650.026.4933741
GATCCAG134450.026.0707321
GATCCTG98800.025.8020631
GATCCCG58550.025.7170731
GATCGTG95300.025.463631
GATCACA198500.024.538421
GATCGCC53150.024.5086651
GATCCCA97150.024.2938921
GATCGCA72500.024.0048161
GATCCAT186800.023.6946451
GATCCAA231200.023.5820081
GATCCTC131150.023.4960231
GATCGTC111500.023.0516071
Produced by FastQC (version 0.11.5)