FastQCFastQC Report
Fri 21 Feb 2020
H33NFAFX2_n01_DJ59.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH33NFAFX2_n01_DJ59.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23048288
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCGC42150.045.861041
GATCCGG44500.041.7078931
GATCCCG45750.039.6498071
GATCGCG27050.039.6147161
GATCTGC74350.039.4698451
GATCCGA78000.038.8799481
GATCCTG73550.038.2327271
GATCCAG83750.038.0503731
GATCTCG74950.037.1447871
GATCTGG61650.036.467361
GATCGTG69800.036.373491
GATCGGC53300.036.332921
GATCACG51950.036.1985441
GATCAGC75600.035.667391
GATCCGT52800.035.549481
GATCTTG101250.035.382051
GATCGCC39250.034.974281
GATCGCA52700.034.8859941
GATCGGG35650.034.6750871
GATCTCC83900.034.392811
Produced by FastQC (version 0.11.5)