FastQCFastQC Report
Sat 9 Mar 2019
H33HLBGXB_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH33HLBGXB_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30171810
Sequences flagged as poor quality0
Sequence length49
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13151574.358893284824477No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT1325480.43931073409251886No Hit
TTCTAGCTCTAAAACCGCCGTTAAGCGGAAACGATCCCCATATAAGAAA906630.30048909893042547No Hit
GCATAGCTCTTAAACTTGAACGGGCCGCGGAAGCGCCCCATATAAGAAA736030.2439462531415914No Hit
GCATAGCTCTTAAACCTTGTCCTTGAACTCTATTCCCCCATATAAGAAA710900.23561728646706975No Hit
GCATAGCTCTTAAACCTGTCTATGGAGACTTTAGCCCCCATATAAGAAA611940.202818458687099No Hit
TTCTAGCTCTAAAACCATATAGGTCCTTGGGAACCCCCCATATAAGAAA597170.19792316072519348No Hit
GCATAGCTCTTAAACCGCCGTTAAGCGGAAACGATCCCCATATAAGAAA487480.16156803320715596No Hit
TTCTAGCTCTAAAACCGGTGAACAGCTCCTCGCCCCCCATATAAGAAAC315490.10456449248487246No Hit
TTCTAGCTCTAAAACCGGTGAACAGCTCCTCGCCCCCCATATAAGAAAA310170.10280125720001551No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAGC592900.042.2829781
ATAGCTC631500.042.0196343
TAGCTCT1023800.042.0164224
AACCGGT175600.041.84912513
TTCTAGC385800.041.7222561
CATAGCT604150.041.6797142
CTAGCTC398700.041.6722373
GCCGTTA182650.041.56257617
ACGATCC184350.041.25895331
TCTAGCT399550.041.1255232
TGTCCGA159000.041.12047213
ACCGGTG179000.041.05476814
CCGTTAA184750.041.02195418
GTCCGAC160500.040.8572714
AACGATC187200.040.7684930
CGACAAT163750.040.622217
GTTAAGC188250.040.53330220
CGATCCC189400.040.34013732
CCGACAA171150.040.24740216
TTGTCCG164000.040.2073212