FastQCFastQC Report
Sat 9 Mar 2019
H33HLBGXB_n02_5P_R-HTO.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH33HLBGXB_n02_5P_R-HTO.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8857759
Sequences flagged as poor quality0
Sequence length49
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1216811.3737221796167631No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACA1572250.042.7583431
CATGTTA853100.042.7182582
GTCTAAC1369250.042.6709374
TCACATC1573350.042.6675223
TTACTAT960650.042.660264
ATCACAT1578850.042.659132
ACTGTCT1371750.042.6544081
CATCGGT1571150.042.650966
CTTACTA960100.042.641673
ATCGGTC1568200.042.6328057
TCGGTCC1560700.042.625848
TGAGCTA1437500.042.6083076
CGGTCCC1557950.042.5939189
GAGGCTG1440150.042.576661
TAACGGC1364700.042.575077
GGCTGAG1438600.042.5620883
TACTATC960650.042.5508425
GAGCTAC1437500.042.5396967
TATCCCC950100.042.5366448
GCTGAGC1439250.042.5269174