FastQCFastQC Report
Sat 9 Mar 2019
H33HLBGXB_n02_5P_R-GDO_lenti.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH33HLBGXB_n02_5P_R-GDO_lenti.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10994522
Sequences flagged as poor quality0
Sequence length49
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTAGCTCTAAAACCGGTGAACAGCTCCTCGCCCCCCATATAAGAAAC232940021.186914719894144No Hit
TTCTAGCTCTAAAACCGGTGAACAGCTCCTCGCCCCCCATATAAGAAAA229505420.87452278507424No Hit
TTCTAGCTCTAAAACCGGTGAACAGCTCCTCGCCCCCCATATAAGAAAT196636017.8849066835284No Hit
TTCTAGCTCTAAAACCGGTGAACAGCTCCTCGCCCCCCATATAAGAAAG192472317.506199905734874No Hit
TTCTAGCTCTAAAACCGGTGAACAGCTCCTCGCCCCCCCATATAAGAAA6775506.162614436534849No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1035220.941577996751473No Hit
TTCTAGCTCTAAAACCGGTGAACAGCTCCTCGCCCCCATATAAGAAACC390770.3554224549280087No Hit
TTCTAGCTCTAAAACCGGTGAACAGCTCCTCGCCCCCATATAAGAAACG353080.3211417467717105No Hit
TTCTAGCTCTAAAACCGGTGAACAGCTCCTCGCCCCCATATAAGAAAGC337190.3066890948055768No Hit
TTCTAGCTCTAAAACCGGTGAACAGCTCCTCGCCCCCATATAAGAAACA287180.2612028062702499No Hit
TTCTAGCTCTAAAACCGGTGAACAGCTCCTCGCCCCCATATAAGAAAGG266720.24259353885507712No Hit
TTCTAGCTCTAAAACCGGTGAACAGCTCCTCGCCCCCATATAAGAAATG263300.23948289884726232No Hit
TTCTAGCTCTAAAACCGGTGAACAGCTCCTCGCCCCCATATAAGAAAAG257210.23394377672808334No Hit
TTCTAGCTCTAAAACCGGTGAACAGCTCCTCGCCCCCATATAAGAAAGA236340.21496159632951756No Hit
TTCTAGCTCTAAAACCGGTGAGAAGCATCGTGTCAAAGCACCGACTCGG235820.2144886335213118No Hit
TTCTAGCTCTAAAACCGGTGAACAGCTCCTCGCCCGCCCATATAAGAAA226980.20644826578181388No Hit
TTCTAGCTCTAAAACCGGTGAACAGCTCCTCGCCCCCATATAAGAAAAC220970.20098190717158962No Hit
TTCTAGCTCTAAAACCGGTGAACAGCTCCTCGCCCCCCCCATATAAGAA219520.19966306857178512No Hit
TTCTAGCTCTAAAACCGGTGAACAGCTCCTCGCCCCCATATAAGAAATA214270.19488796329663083No Hit
TTCTAGCTCTAAAACCGGTGAACAGCTCCTCGCCCCCATATAAGAAACT211320.19220480890392505No Hit
TTCTAGCTCTAAAACCGGTGAACAGCTCCTCGCCCCCATATAAGAAAGT195600.17790677939432017No Hit
TTCTAGCTCTAAAACCGGTGAACAGCTCCTCGCCCCCATATAAGAAATC169150.15384934424616187No Hit
TTCTAGCTCTAAAACCGGTGAACAGCTCCTCGCCCCCATATAAGAAAAA154980.1409611077225549No Hit
TTCTAGCTCTAAAACCGGTGAACAGCTCCTCGCCCCCATATAAGAAAAT125400.1140568002865427No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG255.089479E-543.002643
AGGAGAC503.6379788E-1143.002643
AGGAAGT351.7333696E-743.002643
GAGAAAT800.043.002643
GGGAACT208.798716E-443.002643
ACTCGGT401.0193617E-843.002643
ATGAATT255.089479E-543.002643
GCAGACG208.798716E-443.002643
AGGAATT1100.043.00259843
ACCGTTG8350.043.00201814
TACCCGG208.799307E-443.00201413
ACCGGAT255.089889E-543.00201414
ACGTTGA600.043.00201415
GACCGGT7200.043.00201413
CCGGACA551.8189894E-1243.00201415
ACAGGTT302.9622151E-643.00201414
AACATGT650.043.00201413
AACGCTG551.8189894E-1243.00201414
ACGCTGA503.6379788E-1143.00201415
ACATGTG401.0193617E-843.00201414
Produced by FastQC (version 0.11.5)