FastQCFastQC Report
Sat 9 Mar 2019
H33HLBGXB_n02_5P_R-GDO_CITE.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH33HLBGXB_n02_5P_R-GDO_CITE.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14350473
Sequences flagged as poor quality0
Sequence length49
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATAGCTCTTAAACCAATGTTTTCTGGAGGCCCGCCCCATATAAGAAA12316658.582748457141449No Hit
GCATAGCTCTTAAACCCTTTCTGATATCCAGTTTACCCCATATAAGAAA9644646.720781956106952No Hit
GCATAGCTCTTAAACCCGTGTAGCGCAGCTGCTTCCCCCATATAAGAAA9379396.53594484307242No Hit
GCATAGCTCTTAAACCCATTACCAGTTGCTCACTGCCCCATATAAGAAA7993385.570116051227022No Hit
GCATAGCTCTTAAACCTTCATTTCTCAGCAGGGTGCCCCATATAAGAAA7540035.2542031192978795No Hit
GCATAGCTCTTAAACTTGCGACGCTTAGCCTCCGTCCCCATATAAGAAA6330444.411311041803291No Hit
GCATAGCTCTTAAACCGTCATCGTTGAGGTGTTTACCCCATATAAGAAA6150174.285691489054054No Hit
GCATAGCTCTTAAACCACCTCTCAATGCCGTGGTTCCCCATATAAGAAA3662582.552236431509958No Hit
GCATAGCTCTTAAACCGTGGTGAATGGTTTCAGGTCCCCATATAAGAAA3489422.4315714192835314No Hit
GCATAGCTCTTAAACTGTATGTGCTCATTGACCGTCCCCATATAAGAAA2803001.9532457222838577No Hit
GCATAGCTCTTAAACCTTTGTTGTGGTGACAGGTCCCCCATATAAGAAA2699221.8809275485205261No Hit
GCATAGCTCTTAAACCATGTCTCGCCCCGATATGGCCCCATATAAGAAA2396341.6698683032956474No Hit
GCATAGCTCTTAAACCCTGCTAATGTTGAGCCTGGCCCCATATAAGAAA2319551.6163578719670075No Hit
GCATAGCTCTTAAACCCAGAAGGGTCCACGTATCCCCCCATATAAGAAA2182651.5209603195657733No Hit
GCATAGCTCTTAAACTGGGCAAGCTCTACGGCAACCCCCATATAAGAAA1983261.382017164172916No Hit
GCATAGCTCTTAAACTTGAACGGGCCGCGGAAGCGCCCCATATAAGAAA1971391.3737456598120494No Hit
GCATAGCTCTTAAACCACATGACAGGACTTCAGCTCCCCATATAAGAAA1751691.2206496608160582No Hit
GCATAGCTCTTAAACCATTCCTTGATTACAGCTGCCCCATATAAGAAAC1709361.1911523752562023No Hit
GCATAGCTCTTAAACCATTCCTTGATTACAGCTGCCCCATATAAGAAAA1685071.1742261039061221No Hit
GCATAGCTCTTAAACCAGCAATTTCTGGCTGGTTGCCCCATATAAGAAA1633891.138561774235595No Hit
GCATAGCTCTTAAACCAGAAAACTTGCTCTCTGTCCCCCATATAAGAAA1592681.1098449507552817No Hit
GCATAGCTCTTAAACCATTCCTTGATTACAGCTGCCCCATATAAGAAAT1464751.0206980634018126No Hit
GCATAGCTCTTAAACCATTCCTTGATTACAGCTGCCCCATATAAGAAAG1420580.9899185901398511No Hit
GCATAGCTCTTAAACTGTCTATTCCATCTACGTCCCCCCATATAAGAAA1248580.8700619136386655No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1189870.8291503701654992No Hit
GCATAGCTCTTAAACTTGATTTGTCAAGGCAGTGCCCCCATATAAGAAA1159070.8076876629780775No Hit
GCATAGCTCTTAAACAATAAACACTATACCATGTACCCCATATAAGAAA1137060.7923501894327803No Hit
GCATAGCTCTTAAACTTCTGATATCCAGTTTAGGTCCCCATATAAGAAA1105390.7702812304514284No Hit
GCATAGCTCTTAAACCACTTTAGATTCATTGATGCCCCCATATAAGAAA1018760.7099138822810928No Hit
GCATAGCTCTTAAACCTCCTTTTCTTTGGTCTTACCCCCATATAAGAAA1016510.708345989710583No Hit
GCATAGCTCTTAAACCCACGGCCTTTTTTTGTTATCCCCATATAAGAAA951870.6633021782626956No Hit
GCATAGCTCTTAAACCCTTCGTGTACGGTTGCACACCCCATATAAGAAA857960.597861826575333No Hit
GCATAGCTCTTAAACTGGAGGAGACAATACCCTCACCCCATATAAGAAA828810.5775489072729519No Hit
GCATAGCTCTTAAACCTGCATCAGATCTCTGCCATCCCCATATAAGAAA794630.5537308770240534No Hit
GCATAGCTCTTAAACCCGGAGTATCCGTTCAAGCCCCCCATATAAGAAA789690.5502884817803566No Hit
GCATAGCTCTTAAACCACAAGTTTTGATTCGGACACCCCATATAAGAAA773950.5393202021982133No Hit
GCATAGCTCTTAAACCCAGATGAATTCTGAACTGTCCCCATATAAGAAA736070.5129238597222544No Hit
GCATAGCTCTTAAACCTTGTCCTTGAACTCTATTCCCCCATATAAGAAA708320.49358651801930153No Hit
GCATAGCTCTTAAACCCACATAAAGTACATTGTGACCCCATATAAGAAA655800.45698842121789296No Hit
GCATAGCTCTTAAACCCGTAGTGGGGCTGTACACCCCCCATATAAGAAA546910.3811093892166481No Hit
GCATAGCTCTTAAACCGCAGTGGACCTGCAAAATCCCCCATATAAGAAA522650.3642040231008413No Hit
GCATAGCTCTTAAACCACGTGTCTACACTTAGCCTCCCCATATAAGAAA521680.36352808719266605No Hit
GCATAGCTCTTAAACCCCCAGTGTTGTCCTGTAGACCCCATATAAGAAA503350.35075498905158037No Hit
GCATAGCTCTTAAACGGACCATCTTTCCCGGAGTCCCCCATATAAGAAA501100.34918709648107066No Hit
GCATAGCTCTTAAACCAGGTATGGGGGAGGCTTCTCCCCATATAAGAAA435080.30318164425660393No Hit
GCATAGCTCTTAAACCTGTCTATGGAGACTTTAGCCCCCATATAAGAAA401690.27991411851024006No Hit
GCATAGCTCTTAAACCGCCGTTAAGCGGAAACGATCCCCATATAAGAAA398190.2774751745116694No Hit
GCATAGCTCTTAAACCTGTCACGGACTCTGCACCCCCCCATATAAGAAA377770.26324567838286583No Hit
GCATAGCTCTTAAACCTTCTTGCGTCTCCGGCCGGCCCCATATAAGAAA375850.26190774338936423No Hit
GCATAGCTCTTAAACCCGTGGTGACGTCCGGAGGGCCCCATATAAGAAA353380.24624972291854072No Hit
GCATAGCTCTTAAACCTCTGCCTGATGACCACAGCCCCCATATAAGAAA340180.23705141983821715No Hit
GCATAGCTCTTAAACCGTATCCATCAACAGTAACACCCCATATAAGAAA330330.23018753458509694No Hit
GCATAGCTCTTAAACCCTGCTGTGGGATTACGTGTCCCCATATAAGAAA300360.20930320554590778No Hit
GCATAGCTCTTAAACCCAGCATGTTGTACCAAAGGCCCCATATAAGAAA299180.20848093299781828No Hit
GCATAGCTCTTAAACTTTTTGTCGGCATCTTGAAGCCCCATATAAGAAA290470.20241144664708963No Hit
GCATAGCTCTTAAACCATTCCTTGGATTACAGCTGCCCCATATAAGAAA286090.1993592824431641No Hit
GCATAGCTCTTAAACCCTTTCTGATATCCAGTTTACCCCCATATAAGAA275310.1918473349275665No Hit
GCATAGCTCTTAAACGTAGAGAGCGGCGCGCCTACCCCCATATAAGAAA239300.16675408538798686No Hit
GCATAGCTCTTAAACCCGTGTAGCGCAGCTGCTTCCCCCCATATAAGAA238540.16622448611972582No Hit
GCATAGCTCTTAAACCCTTGCAGGACGGCAGAGCTCCCCATATAAGAAA229290.15977870555207485No Hit
GCATAGCTCTTAAACCCGCACTTTAGATTCATTGACCCCATATAAGAAA197010.13728467347382906No Hit
GCATAGCTCTTAAACGAAGAAGCTGTTTCAGCTGTCCCCATATAAGAAA194920.13582827548611115No Hit
GCATAGCTCTTAAACCCCTACCACCTGAAAGCCTTCCCCATATAAGAAA194470.1355146969720092No Hit
GCATAGCTCTTAAACCGTGGTGAATGGTTTCAGGTCCCCCATATAAGAA189020.1317169127456635No Hit
GCATAGCTCTTAAACCCGTGTAGCGCAGCTGCTTCCCCATATAAGAAAC186470.12993996783241918No Hit
GCATAGCTCTTAAACCACCTTCAAGCAACGCCGCACCCCATATAAGAAA185610.12934068444991326No Hit
GCATAGCTCTTAAACAGTCTACGTATTTGCCCCCACCCCATATAAGAAA181040.12615612042892244No Hit
GCATAGCTCTTAAACCGTCATCGTTGAGGTGTTTACCCCCATATAAGAA170490.11880444637608809No Hit
GCATAGCTCTTAAACCCGTGTAGCGCAGCTGCTTCCCCATATAAGAAAG161710.11268618114538803No Hit
GCATAGCTCTTAAACCCCAGTACACCTTGCACTTGCCCCATATAAGAAA159280.11099285716923755No Hit
GCATAGCTCTTAAACCCTTTCTGATATCCAGTTTACCCATATAAGAAAG158890.11072108912368255No Hit
GCATAGCTCTTAAACCCTTTCTGATATCCAGTTTACCCATATAAGAAAC158680.11057475248376832No Hit
GCATAGCTCTTAAACCCGTGTAGCGCAGCTGCTTCCCCATATAAGAAAA147600.1028537526254361No Hit
GCATAGCTCTTAAACTGACAGAGGAGAAGTTCACACCCCATATAAGAAA146640.10218478512868531No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGCG503.6379788E-1143.0043213
GCAATGT255.0883595E-543.0043215
ACACGGG5050.043.00431414
ACCGCTA4500.043.00431414
ACATTTG600.043.00431414
CCAATCA551.8189894E-1243.00431415
CGTTCAT302.9611892E-643.00431415
ACCTCGG302.9611892E-643.00431414
ATCGTCA208.7970786E-443.00431414
CTAGGTA551.8189894E-1243.00431415
AATCGTC208.7970786E-443.00431413
ACGGTTT208.7970786E-443.00431414
CTAGCGA600.043.00431415
CACCCGT1050.043.0043113
TTTCGGA401.0191798E-843.00341818
CAGTTCT1350.043.00341819
GTCTCGA401.0191798E-843.00341819
TCCTAAT401.0191798E-843.00341818
TCGTCGT950.043.00341818
TGCCTGA39850.043.00341819
Produced by FastQC (version 0.11.5)