FastQCFastQC Report
Sat 9 Mar 2019
H33HLBGXB_n02_5P_R-BLG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH33HLBGXB_n02_5P_R-BLG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16478562
Sequences flagged as poor quality0
Sequence length49
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3693352.2413060071625184No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC2856550.042.631211
TGTCCTA2247750.042.59116411
CAACGCT2857250.042.58764612
AACGCTT2860550.042.557213
GTCCTAC2248050.042.55104412
TCCTACC2240550.042.5386713
ATGTCCT556000.042.50254410
TTGTCCG5416300.042.48189512
GTCTTTG4762200.042.48050311
TGTCCGA5412350.042.4777913
GTCAACG1343250.042.47157710
CTCAACG738950.042.4689310
AGTCTTT1464600.042.4670310
TCCGACA5419500.042.45849615
GTCCGAC5413550.042.44876514
CGTCTTT905700.042.4458910
CGCTTGG2858300.042.42811215
CTGTCCT591250.042.394110
CCGACAA5428100.042.39170516
TCTTTGT4770450.042.37954712