FastQCFastQC Report
Sat 9 Mar 2019
H33HLBGXB_n02_5P_G-HTO.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH33HLBGXB_n02_5P_G-HTO.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9794472
Sequences flagged as poor quality0
Sequence length49
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG456290.4658648266083154No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGTTA1353100.042.7260482
TATCACA1663600.042.7101551
CATCGGT1657300.042.6794246
TCACATC1661100.042.6792833
ATCGGTC1653550.042.6730777
GGCTGAG1639350.042.6645853
TCGGTCC1644700.042.637788
GAGGCTG1642100.042.634371
ATCACAT1669200.042.607192
CGGTCCC1642650.042.5921679
CTTACTA1092950.042.5832983
AGCTTAC1092750.042.5635381
GGTCCCA2299450.042.5474910
ATGTTAC1358850.042.5438843
CCGTCCC1327700.042.53749
TATCCCC1077850.042.531818
TGTTACC1346800.042.501534
GTTACCG1342650.042.4954155
TGAGCTA1647650.042.493596
CGTATTC742850.042.4829647