FastQCFastQC Report
Sat 9 Mar 2019
H33HLBGXB_n02_5P_G-GDO_lenti.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH33HLBGXB_n02_5P_G-GDO_lenti.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13362588
Sequences flagged as poor quality0
Sequence length49
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTAGCTCTAAAACCGCCGTTAAGCGGAAACGATCCCCATATAAGAAA634257047.465131754417634No Hit
TTCTAGCTCTAAAACCATATAGGTCCTTGGGAACCCCCCATATAAGAAA425748931.861260707880838No Hit
TTCTAGCTCTAAAACCATATAGGTCCTTGGGAACCCCCCCATATAAGAA2737132.048353208225832No Hit
TTCTAGCTCTAAAACCATATAGGTCTTTGGGAACCCCCCATATAAGAAA2165141.620299899989433No Hit
TTCTAGCTCTAAAACCGCCGTTAAGCGGAAACGATCCCATATAAGAAAC1190000.8905460529053204No Hit
TTCTAGCTCTAAAACCGCCGTTAAGCGGAAACGATCCCATATAAGAAAG1036040.7753288509680909No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG832610.6230903774029403No Hit
TTCTAGCTCTAAAACCGCCGTTAAGCGGAAACGATCCCATATAAGAAAT690010.5163745226598321No Hit
TTCTAGCTCTAAAACCATATAGGTCCTTGGGAACCCCCATATAAGAAAG687320.5143614395654494No Hit
TTCTAGCTCTAAAACCATATAGGTCCTTGGGAACCCCCATATAAGAAAC682180.5105148793033206No Hit
TTCTAGCTCTAAAACCGCCGTTAAGCGGAAACGATCCCATATAAGAAAA674080.5044531792793432No Hit
TTCTAGCTCTAAAACCGCCGTTAAGCGGAAACGATCCCCCATATAAGAA595310.44550501744123217No Hit
TTCTAGCTCTAAAACCATATAGGTCCTTGGGAACCCCCATATAAGAAAT515460.38574862893325756No Hit
TTCTAGCTCTAAAACCATATAGGTCCTTGGGAACCCCCATATAAGAAAA501700.3754512224727725No Hit
TTCTAGCTCTAAAACCGCCGTTAAGCGGAAACGATCGCCCATATAAGAA348440.26075787115489907No Hit
TTCTAGCTCTAAAACCATATAGGTCTTTGGGAACCCCCCCATATAAGAA157540.11789632367622199No Hit
TTCTAGCTCTAAAACCATATAGGTCCTTGGGACCCCCCCATATAAGAAA143430.10733699190605892No Hit
TTCTAGCTCTAAAACCATATAGGTCCTTAGGAACCCCCCATATAAGAAA140860.10541371177499449No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCCGA302.961424E-643.00378815
CAGCACT302.961424E-643.00378815
CCGCCTT3150.043.00378815
CAGCCTT401.0189979E-843.00378415
CCCACGT208.797591E-443.00378415
CCGACTT800.043.00378415
ACGCCGA503.6379788E-1143.0037815
CAGCCGT8800.043.0037815
CCTACCT255.0887127E-543.0037815
CCTGTTT456.020855E-1043.0037815
CCGACGA255.0887127E-543.0037815
CACCCGT255.0887127E-543.0037815
ACCGGTG351.7330785E-743.00362414
CATGCCC1000.043.00362414
ACCTCAG351.7330785E-743.00362414
ACGATTT255.088825E-543.00362414
ACCAGGC255.088825E-543.00362414
ACCGCTG5150.043.0036214
ACCGCCT3250.043.0036214
ACCGTAT1450.043.0036214
Produced by FastQC (version 0.11.5)