FastQCFastQC Report
Sat 9 Mar 2019
H33HLBGXB_n02_5P_G-GDO_CITE.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH33HLBGXB_n02_5P_G-GDO_CITE.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16232789
Sequences flagged as poor quality0
Sequence length49
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATAGCTCTTAAACCTTGTCCTTGAACTCTATTCCCCCATATAAGAAA305990818.850168014874093No Hit
GCATAGCTCTTAAACTTGAACGGGCCGCGGAAGCGCCCCATATAAGAAA305866618.842516834291384No Hit
GCATAGCTCTTAAACCTGTCTATGGAGACTTTAGCCCCCATATAAGAAA265286616.342638347606194No Hit
GCATAGCTCTTAAACCGCCGTTAAGCGGAAACGATCCCCATATAAGAAA211107313.00499254933949No Hit
GCATAGCTCTTAAACCCTGCTGTGGGATTACGTGTCCCCATATAAGAAA12537267.723417091172688No Hit
GCATAGCTCTTAAACCATATAGGTCCTTGGGAACCCCCCATATAAGAAA7470244.601944866036268No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2593151.5974765642552242No Hit
GCATAGCTCTTAAACCTTGTCCTTGAACTCTATTCCCCCCATATAAGAA1141470.7031878502209324No Hit
GCATAGCTCTTAAACTTGAACGGGCCGCGGAAGCGCGCCCCATATAAGA941010.5796970563715207No Hit
GCATAGCTCTTAAACCTTGTCCTTGAACTCTATTCCCCATATAAGAAAC772000.475580628812461No Hit
GCATAGCTCTTAAACCTTGTCCTTGAACTCTATTCCCCATATAAGAAAG689290.4246282015986286No Hit
GCATAGCTCTTAAACCTTGTCCTTGAACTCTATTCCCCATATAAGAAAA584070.35980877962499236No Hit
GCATAGCTCTTAAACTTGAACGGGCCGCGGAAGCGCCCCCATATAAGAA575570.3545724644113837No Hit
GCATAGCTCTTAAACCTTGTCCTTGAACTCTATTCCCCATATAAGAAAT522650.3219717819285398No Hit
GCATAGCTCTTAAACCGCCGTTAAGCGGAAACGATCCCATATAAGAAAC449080.2766499336620466No Hit
GCATAGCTCTTAAACCTGTCTATGGAGACTTTAGCCCCCCATATAAGAA397610.24494250495093606No Hit
GCATAGCTCTTAAACCGCCGTTAAGCGGAAACGATCCCATATAAGAAAG393770.24257692254855281No Hit
GCATAGCTCTTAAACTTGAACGGGCCGCGGAAGCGCCCATATAAGAAAC379140.2335643000103063No Hit
GCATAGCTCTTAAACTTGAACGGGCCGCGGAAGCGCCCATATAAGAAAG326090.20088353270654846No Hit
GCATAGCTCTTAAACCATATAGGTCCTTGGGAACCCCCCCATATAAGAA325990.20082192899815304No Hit
GCATAGCTCTTAAACCGCCGTTAAGCGGAAACGATCCCATATAAGAAAT283090.17439393809652795No Hit
GCATAGCTCTTAAACTTGAACGGGCCGCGGAAGCGCGCCCATATAAGAA273860.16870791581163286No Hit
GCATAGCTCTTAAACCCTGCTGTGGGATTACGTGTCCCCCATATAAGAA267660.1648884858911183No Hit
GCATAGCTCTTAAACCGCCGTTAAGCGGAAACGATCCCATATAAGAAAA254900.1570278526998657No Hit
GCATAGCTCTTAAACCGCCGTTAAGCGGAAACGATCCCCCATATAAGAA239220.1473683912234675No Hit
GCATAGCTCTTAAACTTGAACGGGCCGCGGAAGCGCCCATATAAGAAAT238660.14702341045645329No Hit
GCATAGCTCTTAAACCTTGTCCTTGAACTCTATTCCACCCATATAAGAA224020.13800462754736725No Hit
GCATAGCTCTTAAACTTGAACGGGCCGCGGAAGCGCCCATATAAGAAAA209580.12910905205507198No Hit
GCATAGCTCTTAAACCTTGTCCTTGAACTCTATTTCCCCATATAAGAAA199330.12279467194454385No Hit
GCATAGCTCTTAAACCTGTCTATGGAGACTTTAGCCCCATATAAGAAAC194910.12007178803346732No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCATTC302.9619823E-643.0026919
ACTATGC208.7987486E-443.0026919
AGTCCTA255.0895236E-543.0026918
TCTCTGC1100.043.0026919
CAACGGG2250.043.0026918
TGTTCTT850.043.0026918
CTCATTG650.043.0026919
TCTCATT700.043.0026918
TCGAGGG401.0193617E-843.0026919
AGTAAAG255.0895236E-543.0026919
TATCCTT1250.043.0026918
TCTATGT1600.043.0026919
GCCTTGC255.0896164E-543.0025620
TACTTCC255.0896164E-543.0025620
TCATTCA208.7988825E-443.0025620
CATTCAA208.7988825E-443.0025621
TAGTTCA208.7988825E-443.0025620
AGTGCGA208.7988825E-443.0025621
TGATTGA255.0896164E-543.0025620
TATAGAG1100.043.0025621
Produced by FastQC (version 0.11.5)