FastQCFastQC Report
Sat 9 Mar 2019
H33HLBGXB_n02_5P_G-BLG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH33HLBGXB_n02_5P_G-BLG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20035317
Sequences flagged as poor quality0
Sequence length49
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG637590.3182330481718857No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCCTA4301150.042.62805611
TCAACGC3907250.042.61572311
GTCCTAC4301900.042.59745412
TCCTACC4287150.042.5844513
CAACGCT3907450.042.5742412
AACGCTT3911050.042.54372413
ATGTCCT1054000.042.5361510
CTGTCCT1136950.042.52848410
TGTCCGA5713500.042.490813
TTGTCCG5721350.042.49070412
GTCTTTG5087950.042.4605611
GTCAACG1845000.042.45820210
TCCGACA5726750.042.45807615
CGCTTGG3904650.042.4450415
AGTCTTT1566900.042.4386610
GTCCGAC5717800.042.43643614
ACGCTTG3913900.042.4182414
CTCAACG997600.042.4064110
CCGACAA5735850.042.39491716
GTGTCCT1147450.042.38103510